Inhibition of biofilm formation of periodontal pathogens by D-Arabinose
안선진, 남궁종욱, 하경원, 전혜경, 김현영, 최봉규,
소속 상세정보
안선진 ( An Sun-Jin ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
남궁종욱 ( Namkung Jong-Uk ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
하경원 ( Ha Kyung-Won ) - Osstem Implant Co. Ltd. Bone Science R&D Center
전혜경 ( Jun Hye-Kyoung ) - Osstem Implant Co. Ltd. Bone Science R&D Center
김현영 ( Kim Hyun-Young ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
최봉규 ( Choi Bong-Kyu ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
Abstract
Periodontitis and periimplantitis are caused as a result of dental biofilm formation. This biofilm is composed of multiple species of pathogens. Therefore, controlling biofilm formation is critical for disease prevention. To inhibit biofilm formation, sugars can be used to interrupt lectin-involving interactions between bacteria or between bacteria and a host. In this study, we evaluated the effect of D-Arabinose on biofilm formation of putative periodontal pathogens as well as the quorum sensing activity and whole protein profiles of the pathogens. Crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy revealed that D-Arabinose inhibited biofilm formation of Porphyromonas gingivalis , Fusobacterium nucleatum , and Tannerella forsythia . D-Arabinose also significantly inhibited the activity of autoinducer 2 of F. nucleatum and the expression of representative bacterial virulence genes. Furthermore, D-Arabinose treatment altered the expression of some bacterial proteins. These results demonstrate that D-Arabinose can be used as an antibiofilm agent for the prevention of periodontal infections.
키워드
Periodontal pathogens; Biofilm inhibition; D-Arabinose; Quorum sensing
원문 및 링크아웃 정보
등재저널 정보